RIPA Buffer: Difference between revisions
Created page with '==RIPA Buffer (for 10mL lysis buffer)== {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- |Tris pH7.4 || 50mM || 500uL || 1M |- |Na Deoxycholate || 0....' |
changed vanadate concentration and volume |
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! || Final Concentration || per 10 mL || Stock || Location | ! || Final Concentration || per 10 mL || Stock || Location | ||
|- | |- | ||
|Tris pH7.4 || 50mM || 500uL || 1M | |Tris pH7.4 || 50mM || 500uL || 1M || Shelf | ||
|- | |- | ||
|Na Deoxycholate || 0.25% || 250uL || 10% | |Na Deoxycholate || 0.25% || 250uL || 10% || Shelf | ||
|- | |- | ||
| NP-40 || 1% || 1mL || 10% | | NP-40 || 1% || 1mL || 10% || Tingting | ||
|- | |- | ||
|NaCl || 150mM || 375uL || 4M | |NaCl || 150mM || 375uL || 4M || Shelf | ||
|- | |- | ||
|EDTA || 1mM || 20uL || 0.5M | |EDTA || 1mM || 20uL || 0.5M || Shelf | ||
|- | |- | ||
|NaVO3 || | |NaVO3 || 100uM || 10uL || 100mM || Biochemical Stocks (-20) | ||
|- | |- | ||
|NaF || 5mM || 100uL || 500mM | |NaF || 5mM || 100uL || 500mM || Shelf | ||
|- | |- | ||
|NaPPi || 25 mM || 1 mL || 250mM | |NaPPi || 25 mM || 1 mL || 250mM || Shelf | ||
|- | |- | ||
| Protease Inhibitors || || 1 mini tablet || || 4C | | Protease Inhibitors || || 1 mini tablet || || 4C | ||
|} | |} | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||