Difference between revisions of "Nr3c1 Genotyping"

From Bridges Lab Protocols
Jump to: navigation, search
(added cre primers)
Line 1: Line 1:
 
==PRIMERS==
 
==PRIMERS==
 +
nr3c1
 
*Fwd: AAACAGGGTTATGCTTGGCA
 
*Fwd: AAACAGGGTTATGCTTGGCA
 
*Rev: TGCCTGCTAGGCAAATGATCT
 
*Rev: TGCCTGCTAGGCAAATGATCT
  
Make primer dilution at 1uM (10uL each primer + 980uL water). Primers in '''Genotyping Box'''  are marked with a star.
+
Cre
 +
*Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
 +
*Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG
 +
 
 +
 
 +
Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in '''Genotyping Box'''  are marked with a star.
  
 
[[ Category:Genotyping ]]
 
[[ Category:Genotyping ]]

Revision as of 15:10, 5 June 2020

PRIMERS

nr3c1

  • Fwd: AAACAGGGTTATGCTTGGCA
  • Rev: TGCCTGCTAGGCAAATGATCT

Cre

  • Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
  • Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG


Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in Genotyping Box are marked with a star.