162
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Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
* Alternatively, if multiple immunoprecipitations will be performed from the same chromatin preparation, use the appropriate volume of Protein G Agarose for the correct number of immunoprecipitations.
20. Incubate for 1 hour at 4°C with rotation.
21. Pellet agarose by brief centrifugation (3000-5000 x g for 1 minute).
* Do not spin Protein G Agarose beads at high speeds. Applying excessive g-force may crush or deform the beads and cause them to pellet inconsistently.
22. Remove 10 μL (1%) of the supernatant as Input and save at 4°C until Section D, step 1.