Preparing Cell Lysates: Difference between revisions
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# Stimulate cells if necessary | # Stimulate cells if necessary | ||
# Wash cells 2x1mL with ice cold PBS -/- and aspirate | # Wash cells 2x1mL with ice cold PBS -/- and aspirate | ||
# Add 200uL [[Buffer | # Add 200uL [[Buffer:RIPA]] buffer and scrape cells | ||
# Pipet into cold eppendorf tubes | # Pipet into cold eppendorf tubes | ||
# rotate end over end for 30 minutes at 4C to lyse | # rotate end over end for 30 minutes at 4C to lyse | ||