Preparing Cell Lysates: Difference between revisions
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===RIPA Buffer (for 10mL lysis buffer)=== | ===RIPA Buffer (for 10mL lysis buffer)=== | ||
{border = "1" | {| border="1" | ||
|-Tris pH7.4 | ! || Final Concentration || per 10 mL || Stock | ||
|-Na Deoxycholate | |- | ||
|-NP-40 | |Tris pH7.4 || 50mM || 500uL || 1M | ||
|-NaCl | |- | ||
|-EDTA | |Na Deoxycholate || 0.25% || 250uL || 10% | ||
|-NaVO3 | |- | ||
|-NaF | | NP-40 || 1% || 1mL || 10% | ||
|-Protease Inhibitors !! 1 tab | |- | ||
} | |NaCl || 150mM || 375uL || 4M | ||
|- | |||
|EDTA || 1mM || 20uL || 0.5M | |||
|- | |||
|NaVO3 || 1mM || 100uL || 1M | |||
|- | |||
|NaF || 5mM || 100uL || 250mM | |||
|- | |||
|NaPPi || 25 mM || 1 mL || 500mM | |||
|- | |||
|- Protease Inhibitors !! 1 tab | |||
|} | |||
==Basic Protocol== | ==Basic Protocol== | ||