Real Time PCR From Cell Culture: Difference between revisions
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===Plate Preparation=== | ===Plate Preparation=== | ||
#Book 3h on qPCR machine at http://felix2.lsi.umich.edu/ORS | #Book 3h on qPCR machine at http://felix2.lsi.umich.edu/ORS | ||
#Prepare primer mix from 5 uM primer pair stocks by adding 0.4 uL primers + 4.6 uL water per reaction | #Prepare primer mix from 5 uM primer pair stocks by adding 0.4 uL primers + 4.6 uL water per reaction | ||
#Get 96 well block and keep on rack. Do not touch bottom of plate. | #Get 96 well block and keep on rack. Do not touch bottom of plate. | ||