Real Time PCR From Cell Culture: Difference between revisions
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*SyberGreen PCR Master Mix Applied Biosystems | *SyberGreen PCR Master Mix Applied Biosystems | ||
*96 well qPCR plate | *96 well qPCR plate | ||
*Primers (Dilute to | *Primers (Dilute to 5 uM mixture of fwd and rev and then make a working solution of 0.4 uL + 4.6 uL water per reaction) | ||
*Generate primers using http://pga.mgh.harvard.edu/primerbank/index.html | *Generate primers using http://pga.mgh.harvard.edu/primerbank/index.html | ||