Real Time PCR From Cell Culture: Difference between revisions
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#Use Superscript RT-PCR kit from Invitrogen, following manufacturers instructions | #Use Superscript RT-PCR kit from Invitrogen, following manufacturers instructions | ||
#Store cDNA at -20 until use | #Store cDNA at -20 until use | ||
#Typically dilute 5X in water (adjust for higher or lower expressing transcripts) | |||
===Plate Preparation=== | ===Plate Preparation=== | ||