Seahorse XF Media: Difference between revisions
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This minimal media stock is the base media for both the glycolysis and mitochondrial stress assays. It contains no buffering and no carbon sources. It is a non-buffered media but still needs to be brought to pH 7.4 and checked before each assay. | This minimal media stock is the base media for both the glycolysis and mitochondrial stress assays. It contains no buffering and no carbon sources. It is a non-buffered media but still needs to be brought to pH 7.4 and checked before each assay. | ||
For glycolysis assays add: | For glycolysis assays add to warmed XF base media just before the assay and recheck pH: | ||
* 1-4 mM L-Glutamine | * 1-4 mM L-Glutamine (Generally 2 mM is 1mL/100mL of 200 mM stock) | ||
For mitochondrial assays add: | For mitochondrial assays and cellular phenotype assays add to warmed XF base media just before the assay and recheck pH: | ||
* 0.5-10 mM Sodium pyruvate | * 0.5-10 mM Sodium pyruvate (Generally 1 mM is 100uL/100mL of 1M stock) | ||
* 1-4 mM L-Glutamine | * 1-4 mM L-Glutamine (Generally 2 mM is 1mL/100mL of 200 mM stock) | ||
* 2- | * 2-25 mM Glucose (Generally 10 mM is 1mL/100mL of 1M stock) | ||
To make base media add the following: | To make base media add the following: | ||