TaqMan SNP Assay: Difference between revisions
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# Dilute gDNA samples to 20ng/ul approx. | # Dilute gDNA samples to 20ng/ul approx. | ||
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | ||
# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X)). | # Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below). | ||
# Add Reaction Mix (5.25 ul) added to each well (Table 1.) | # Add Reaction Mix (5.25 ul) added to each well (Table 1.) | ||
# Diluted gDNA samples each added to a single well (Table 1.) | # Diluted gDNA samples each added to a single well (Table 1.) | ||
'''Table 1. | |||
'''Table 1. Full 96 well plate''' | |||
{| class="wikitable" | {| class="wikitable" | ||
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|gDNA Sample (20ng/ul) | |gDNA Sample (20ng/ul) | ||
|4.50 | |4.50 ul | ||
|501.6 | |501.6 ul | ||
|- | |- | ||
! | ! | ||