TaqMan SNP Assay: Difference between revisions

← Older edit

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 == Protocol ==
-# Dilute gDNA samples to  ng/ul.
+# Dilute gDNA samples to 20ng/ul approx.
 # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
-# Make up Reaction Master mix (see table below).
+# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).
+# Add Reaction Mix (5.25 ul) added to each well (Table 1.)
+# Diluted gDNA samples each added to a single well (Table 1.)
-'''Table 1. Reaction Mix for full 96 well plate'''
+'''Table 1. Full 96 well plate'''
 {| class="wikitable"
 |-
@@ Line 34: / Line 36: @@
 |26.4 ul
 |-
-!
+|gDNA Sample (20ng/ul)
-!5.25 ul
+|4.50 ul
-!554.4 ul
-|}
-# Reaction Mix (5.25 ul) added to each well (see Table 2.)
-# Diluted gDNA samples each added to a single well (see Table 2.)
-'''Table 2.  for full 96 well plate'''
-{| class="wikitable"
-|-
-! Component
-! 1X volume
-! 96X Volume (+ 10% excess)
-|-
-|Reaction Master Mix
-|5.25 ul
-|554.4 ul
-|-
-|gDNA samples
-|4.75 ul
 |501.6 ul
 |-