TaqMan SNP Assay: Difference between revisions

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 == Protocol ==
-# Dilute gDNA samples to 1-5 ng/ul.
+# Dilute gDNA samples to 20ng/ul approx.
-# Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice.
+# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
-# Make up Reaction Master mix (see table below).
+# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).
+# Add Reaction Mix (5.25 ul) added to each well (Table 1.)
+# Diluted gDNA samples each added to a single well (Table 1.)
-TO BE COMPLETED
+'''Table 1. Full 96 well plate'''
+{| class="wikitable"
+|-
+! Component
+! 1X volume
+! 96X Volume (+ 10% excess)
+|-
+|TaqMan® Genotyping Master Mix (2X)
+|5.00 ul
+|528.0 ul
+|-
+|TaqMan® Genotyping Assay (40X)
+|0.25 ul
+|26.4 ul
+|-
+|gDNA Sample (20ng/ul)
+|4.50 ul
+|501.6 ul
+|-
+!
+!10 ul
+!1056 ul
+|}