Designing and Generating CRISPR-Cas Mutants: Difference between revisions

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 [[ Category: CRISPR ]]
-==Overview==
-We are using the double-nickase mutation strategy.  This involves the generation of two CRISPR-Cas plasmids per mutation, each of which nicks a different strand, spaced apart by about 40 nt.  To generate a point mutant, you can also include a piece of DNA containing the mutation and allow for homologous recombination.