Paraffin Embedding of Tissue Samples: Difference between revisions

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Paraffin embedding protocol: added note about sending to core
 
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==SOP==
*[[SOP - Carcinogens]]
*[[SOP - Flammables and Combustibles]]
*[[SOP - Formalin and Paraformaldehyde]]
*[[SOP- Irritants]]
== Paraffin embedding protocol ==
== Paraffin embedding protocol ==
#Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample).
#Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample).
#Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required.  
#Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. At this step samples can also be taken to the [https://www.rogelcancercenter.org/research/shared-resources-and-cores/tissue-and-molecular-pathology Rogel Cancer Center's Tissue and Molecular Pathology Core] for the remaining steps
##Note that the subsequent dehydration steps involve many long incubation periods and therefore should be started first thing in the morning (unless you plan on being in the lab overnight!).
##Note that the subsequent dehydration steps involve many long incubation periods and therefore should be started first thing in the morning (unless you plan on being in the lab overnight!).
#Turn on wax pots.
#Turn on wax pots.
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#Store at room temperature until ready to section.
#Store at room temperature until ready to section.


Wash Solution Table
==Wash Solution Table==
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding: 0"
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding: 0"
|-
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|-
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 95% ethanol
|style="border-style: solid; border-width: 0 1px 0 0"| 95% ethanol
|style="border-style: solid; border-width: 0"| 2x 75 min
|style="border-style: solid; border-width: 0"| 75 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 95% ethanol
|style="border-style: solid; border-width: 0"| 75 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 100% ethanol
|style="border-style: solid; border-width: 0"| 60 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 100% ethanol
|style="border-style: solid; border-width: 0"| 60 min
|-
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 100% ethanol
|style="border-style: solid; border-width: 0 1px 0 0"| 100% ethanol
|style="border-style: solid; border-width: 0"| 3x 60 min
|style="border-style: solid; border-width: 0"| 60 min
|-
|-
|style="border-style: solid; border-width: 0 1px 0 0"| Citrosolv
|style="border-style: solid; border-width: 0 1px 0 0"| Citrosolv
|style="border-style: solid; border-width: 0"| 2x 30 min
|style="border-style: solid; border-width: 0"| 30 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| Citrosolv
|style="border-style: solid; border-width: 0"| 30 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 60 degree paraffin
|style="border-style: solid; border-width: 0"| 60 min
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 60 degree paraffin
|style="border-style: solid; border-width: 0"| 60 min
|-
|-
|style="border-style: solid; border-width: 0 1px 0 0"| 60 degree paraffin
|style="border-style: solid; border-width: 0 1px 0 0"| 60 degree paraffin
|style="border-style: solid; border-width: 0"| 3x 60 min
|style="border-style: solid; border-width: 0"| 60 min or O/N
|}
|}