Amitryptyline Treatment of Mice: Difference between revisions
wrote protocol for amitriptyline in water |
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* Makes 1L of drinking water | * Makes 1L of drinking water | ||
* dissolve 0. | * dissolve 0.060g in 1L water | ||
===Calculation=== | ===Calculation=== | ||
* 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg | * 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg drug/kg/mL | ||
* average mouse weighs 0.03kg so want 0.06 mg | * average mouse weighs 0.03kg so want 0.06 mg drug/mL or 60 mg/L | ||
*This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored. | *This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored. | ||