Difference between revisions of "Induction Conditions"

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{| class="wikitable"
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{| class="wikitable" border= "1"
 
|-
 
|-
 
! Protein
 
! Protein
−
! IPTG (uM)
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! IPTG
 
! Time
 
! Time
 
! Temperature
 
! Temperature
 
! Notes
 
! Notes
 
|-
 
|-
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|  some protein
+
|  GST-PLC
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|  some IPTG
+
|  10 uM
−
|  some time
+
|  Overnight
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|  some temperature
+
|  28C
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|  some note
+
|  very soluble and easy to induce.
 
|-
 
|-
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|  row 2, cell 1
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|  GST-HRS
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|  row 2, cell 2
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|  10 uM
−
|  row 2, cell 3
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|  Overnight
 +
|  28C
 +
|  very soluble and easy to induce.
 +
|-
 +
|  GST-HRS (2xFYVE)
 +
|  10 uM
 +
|  Overnight
 +
|  28C
 +
|  very soluble and easy to induce.
 +
|-
 +
|  GST-Rab5
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|  10 uM
 +
|  Overnight
 +
|  28C
 +
|  very soluble and easy to induce.
 
|}
 
|}
 +
[[Category:Protein Purification]]

Latest revision as of 17:54, 24 July 2012

Protein IPTG Time Temperature Notes
GST-PLC 10 uM Overnight 28C very soluble and easy to induce.
GST-HRS 10 uM Overnight 28C very soluble and easy to induce.
GST-HRS (2xFYVE) 10 uM Overnight 28C very soluble and easy to induce.
GST-Rab5 10 uM Overnight 28C very soluble and easy to induce.
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