5' RACE: Difference between revisions
Added PCR reaction |
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# Heat inactivate at 65C for 10min. | # Heat inactivate at 65C for 10min. | ||
=== | ===PCR of dC-Tailed cDNA=== | ||
<ol> | <ol> | ||
<li> Prepare a master mix containing the following (multiply by the number of samples) and aliquot 44.5 uL into PCR tubes.</li> | <li> Prepare a master mix containing the following (multiply by the number of samples) and aliquot 44.5 uL into PCR tubes.</li> | ||