Back-Crossing of Mouse Strains: Difference between revisions
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==Notes== | ==Notes== | ||
* As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can | * As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can either be back-crossed further or intercrossed for maintenance. It is better to keep back-crossing as many times as necessary. | ||
* '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased (see [[Ordering Mice]]) or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research. | * '''Pure Strain''': Make sure that every iteration of a back-cross is to a pure strain, preferably purchased (see [[Ordering Mice]]) or recently purchased from a vender who is careful to maintain the purity of their strains. Generally we will use C57BL6/J (see http://jaxmice.jax.org/strain/000664.html) as it is the most widely available and commonly used strain in diabetes research. | ||
* '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift. | * '''Maintainence of Wild-Type Mice''': Wild-type mice can be purchased from a vendor and carefully inter-crossed. Generations of these mice should be tracked, and after 4 or more generations, a new cohort of wild-type mice should be purchased to avoid any potential genetic drift. | ||