Verifying Kan Deletion Mutants from Genomic DNA: Difference between revisions
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[[Category:Genotyping]] | |||
[[Category:Yeast]] | |||
[[Category:PCR]] | |||
[[Category:DNA]] | |||
==Materials== | ==Materials== | ||
*Order primers to amplify the knockout from the genome. The primers should be ~21 bases long and about 200 to 300 bp upstream from the start and another one that is 200-300 bp from the stop | *Order primers to amplify the knockout from the genome. The primers should be ~21 bases long and about 200 to 300 bp upstream from the start and another one that is 200-300 bp from the stop | ||