Difference between revisions of "French Press"

Latest revision as of 16:14, 6 May 2009

Pour in sample, replace base and place back on the press. Ensure valves are tightened

Set French Press to Down and start. Pour sample into cylinder followed by the piston.
Ensure that the piston is lubricated with glycerol.
Place cylinder with piston into the French Press instrument from the back, making sure that the piston's lever is orthogonal to the brace screws.
Position the valves such that they are accessible to your hands.

For bacteria 15 000 psi of cell pressure is sufficient. For a 1cm cell this corresponds to about 1100 psi on the gauge (see chart)
Turn black knob all the way to the left
Set Press to High and ensure both valves are securely closed
Start the press. The cell will move until it encounters pressure and will stop moving (the motor will still be running)
Slowly turn the knob until the gauge pressure reads desired pressure
Slowly open valve until sample comes out, keep an eye on the pressure and make sure it doesn’t drop. Continue until piston is fully depressed. Close bottom valve
Reload sample if necessary

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 ==Prepare the Cell (Not Auto-Fill)==
-#Invert cell on black stand without base piece.  Remove piston to fill line
 #Pour in sample, replace base and place back on the press.  Ensure valves are tightened
 ==Loading Sample==
-#With the back valve open and the bottom valve closed insert tubing into sample
+#Set French Press to Down and start. Pour sample into cylinder followed by the piston.
-#Set French Press to Down and start.  Suction caused by the piston moving out of the cell will bring sample into the cell
+#Ensure that the piston is lubricated with glycerol.
-#When the base is at the bottom close the intake valve and stop the Press
+#Place cylinder with piston into the French Press instrument from the back, making sure that the piston's lever is orthogonal to the brace screws.
+#Position the valves such that they are accessible to your hands.
 ==Lysing Sample==