Glycogen Determination from Cells: Difference between revisions
added cell lysis as per alan cheng |
clarified source of glycogen |
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# Quantify glucose using kit: | # Quantify glucose using kit: | ||
## Add 1-5 uL glucose standard for standard curve | ## Add 1-5 uL glucose standard for standard curve | ||
## Add 10 uL glycogen. If signal is too low increase the volume. Also make a blank with the same volume of Sodium acetate. | ## Add 10 uL of the glycogen containing sample. If signal is too low increase the volume. Also make a blank with the same volume of Sodium acetate. | ||
## Mix and incubate at 37C for 5 min | ## Mix and incubate at 37C for 5 min | ||
## Measure absorbance at 505 nm | ## Measure absorbance at 505 nm | ||