Yeast Transformation: Difference between revisions
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#Prepare Transformation Mixture for the appropriate number of transformations: | #Prepare Transformation Mixture for the appropriate number of transformations: | ||
{| border="1" | {| border="1" | ||
! | ! Reagent || Concentration || 1 || 5(6X) || 10(11X) | ||
|- | |- | ||
|PEG 3500 || 50% || 240uL || 1440uL || 2640uL | |PEG 3500 || 50% || 240uL || 1440uL || 2640uL | ||
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|Lithium Acetate || 1M || 36uL || 216uL || 396uL | |Lithium Acetate || 1M || 36uL || 216uL || 396uL | ||
|- | |- | ||
|Herring Testes DNA || 10mg/mL || | |Herring Testes DNA || 10mg/mL || 10uL || 60uL || 110uL | ||
|- | |- | ||
|Water|| || 34uL || 204uL || 374uL | |Water|| || 34uL || 204uL || 374uL | ||
|- | |- | ||
|Tube || eppendorf tube || 15mL Falcon Tube || 15 mL Falcon Tube | |Tube || || eppendorf tube || 15mL Falcon Tube || 15 mL Falcon Tube | ||
|} | |} | ||