Preparing a SDS-PAGE Gel: Difference between revisions
added 6% gel |
added SOP requirement-JR |
||
| (2 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
==SOP== | |||
*[[SOP- Electrophoresis]] | |||
==Separating Gel== | ==Separating Gel== | ||
*For 12 mL final volume (2 gels) | *For 12 mL final volume (2 gels) | ||
| Line 16: | Line 19: | ||
| TEMED || 30 uL || 30 uL || 30 uL || 30uL | | TEMED || 30 uL || 30 uL || 30 uL || 30uL | ||
|} | |} | ||
*For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl | *For a [[Phos-Tag Electrophoresis and Western Blotting|Phos-Tag Gel]], add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only | ||
==Stacking Gel== | ==Stacking Gel== | ||
| Line 32: | Line 35: | ||
| Water || 4 mL | | Water || 4 mL | ||
|- | |- | ||
| APS (make fresh, 0.1g/mL in water) | | APS (make fresh, 0.1g/mL in water) || 200 uL | ||
|- | |- | ||
| TEMED || 30 uL | | TEMED || 30 uL | ||
|} | |} | ||
[[Category: SDS-PAGE]] | [[Category: SDS-PAGE]] | ||
[[Category: Electrophoresis]] | [[Category: Electrophoresis]] | ||
[[Category: PhosTag]] | |||
[[Category: Phosphorylation]] | |||