Freezing and Thawing S2 Cells: Difference between revisions
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==SOP== | |||
*[[SOP- Cryogenic Materials]] | |||
*[[SOP- Biosafety Cabinets]] | |||
==METHOD== | |||
#Use and early passage (1-10) of exponentially growing (about ~10^7 cells). | #Use and early passage (1-10) of exponentially growing (about ~10^7 cells). | ||
#Detach by pipeting a stream of media over the cells | #Detach by pipeting a stream of media over the cells | ||
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see PMID 18388942 | see PMID 18388942 | ||
[[Category: Cell Culture]] | |||