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==Materials==* Media( Typically High Glucose DMEM: Pre-prepared 500mL: 1% PSG and 10% PBS). May be different for different cell lines.*C2C12 cellsFrozen cell aliquot in Liquid Nitrogen ==Protocol==* Thaw vile vial of C2C12 cells in 37C water bath approx. 1 min
* Pipette 10mL of media to plate in sterile hood
* Pipette cells from vile into plate with media (evenly pipette/distribute throughout plate)
* Incubate plate in 37C incubator
* Check cells in 4-6 hours for growth (under microscope)
* Replace media after significant growth approx* Passage cells normally, see [[Splitting Cells]]. 85-90%
Notes:
Sanitize surface, vials, hood etc with 70% ethanol and paper towel,