SOP - Autoclave: Difference between revisions
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* Carefully load items to be autoclaved, DO NOT overload the autoclave. | * Carefully load items to be autoclaved, DO NOT overload the autoclave. | ||
* Bottles containing liquids should be placed inside a heat resistant plastic tray (secondary container) containing an inch of water. | * Bottles containing liquids should be placed inside a heat resistant plastic tray (secondary container) containing an inch of water. | ||
* Bottles should not be filled more than 2/3. | |||
* Keep 1-2 inches of space between bottles. | |||
* DO NOT tighten caps on bottles fully. | |||
* Individual glassware pieces should be within a secondary container on a shelf or rack and never placed directly on the autoclave chamber bottom or floor. | * Individual glassware pieces should be within a secondary container on a shelf or rack and never placed directly on the autoclave chamber bottom or floor. | ||
* Make sure that the door of the autoclave is fully closed and latched and ensure that the correct cycle for the items being autoclaved has been selected before starting the cycle. | * Make sure that the door of the autoclave is fully closed and latched and ensure that the correct cycle for the items being autoclaved has been selected before starting the cycle. | ||
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===When autoclaving dry loads:=== | ===When autoclaving dry loads:=== | ||
* Add ¼ to ½ inch of water to the tray so that the bottles will heat evenly. Do not fully tighten bottle caps, this could lead to the bottle shattering. Loose caps allow in inside of the bottle to be sterilized. | |||
* Check plastic materials to ensure that they are compatible with being autoclaved. | * Check plastic materials to ensure that they are compatible with being autoclaved. | ||
* Choose the dry porous materials cycle. | * Choose the dry porous materials cycle. | ||
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I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing <nowiki>* ~~~~</nowiki> in the list below: | I have read and understand the above SOP. I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. Sign by logging in and typing <nowiki>* ~~~~</nowiki> in the list below: | ||
*[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:13, 19 October 2016 (UTC) | *[[User:Mollyec|Mollyec]] ([[User talk:Mollyec|talk]]) 16:13, 19 October 2016 (UTC) | ||
Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? ☐ | * [[User:Davebridges|Davebridges]] ([[User talk:Davebridges|talk]]) 14:53, 8 November 2016 (UTC) | ||
*[[User:Pfeiferl|Pfeiferl]] ([[User talk:Pfeiferl|talk]]) 18:11, 5 June 2017 (UTC) | |||
* [[User:Snyderds|Snyderds]] ([[User talk:Snyderds|talk]]) 19:43, 7 June 2017 (UTC) | |||
*[[User:Reddj|Reddj]] ([[User talk:Reddj|talk]]) 14:04, 9 June 2017 (UTC) | |||
* [[User:Elhabbal|Elhabbal]] ([[User talk:Elhabbal|talk]]) 22:09, 12 June 2017 (UTC) | |||
*[[User:Nlambrec|Nlambrec]] ([[User talk:Nlambrec|talk]]) 21:27, 17 February 2019 (UTC) | |||
*[[User:Kkistler|Kkistler]] ([[User talk:Kkistler|talk]]) | |||
* [[User:Allmeyer|Allmeyer]] ([[User talk:Allmeyer|talk]]) 21:39, 15 January 2020 (UTC) | |||
* [[User:Ccousz|Ccousz]] ([[User talk:Ccousz|talk]]) 13:15, 6 June 2022 (UTC) | |||
Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? X YES ☐ NO | |||
Laboratory Director - Dave Bridges Revision Date - 2016-10-19 | Laboratory Director - Dave Bridges Revision Date - 2016-10-19 | ||