Difference between revisions of "50X TAE Buffer"
From Bridges Lab Protocols
(Created page with "==Reagents== *900mL ddH2O *242g Tris Base *57.1 mL Glacial Acetic Acid *18.6g EDTA 1. Add Tris Base, Glacial Acetic Acid, and EDTA to 900mL water in 1L Erlenmeyer Flask and...") |
Davebridges (Talk | contribs) m (→Reagents: changed category tag) |
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*18.6g EDTA | *18.6g EDTA | ||
| − | 1. Add Tris Base, Glacial Acetic Acid, and EDTA to | + | 1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. |
| + | 2. Add remaining 400mL water and mix on stir plate (or my swirling). | ||
| − | [[Category: | + | [[Category: Buffer]] |
[[Category: Genotyping]] | [[Category: Genotyping]] | ||
Latest revision as of 14:27, 2 August 2017
Reagents
- 900mL ddH2O
- 242g Tris Base
- 57.1 mL Glacial Acetic Acid
- 18.6g EDTA
1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. 2. Add remaining 400mL water and mix on stir plate (or my swirling).
