TaqMan SNP Assay: Difference between revisions
No edit summary |
No edit summary |
||
| Line 16: | Line 16: | ||
# Dilute gDNA samples to 20ng/ul approx. | # Dilute gDNA samples to 20ng/ul approx. | ||
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | ||
# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X)). | # Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below). | ||
# Add Reaction Mix (5.25 ul) added to each well (Table 1.) | # Add Reaction Mix (5.25 ul) added to each well (Table 1.) | ||
# Diluted gDNA samples each added to a single well (Table 1.) | # Diluted gDNA samples each added to a single well (Table 1.) | ||