TaqMan SNP Assay: Difference between revisions

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# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.
# Make up Reaction Master mix (see table below).
# Make up Reaction Master mix (see table below).
 
# Add Reaction Mix (5.25 ul) added to each well (see Table 2.)
# Diluted gDNA samples each added to a single well (see Table 2.)


'''Table 1. Reaction Mix for full 96 well plate'''
'''Table 1. Reaction Mix for full 96 well plate'''
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!554.4 ul
!554.4 ul
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# Reaction Mix (5.25 ul) added to each well (see Table 2.)
# Diluted gDNA samples each added to a single well (see Table 2.)