TaqMan SNP Assay: Difference between revisions
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== Protocol == | == Protocol == | ||
# Dilute gDNA samples to | # Dilute gDNA samples to ng/ul. | ||
# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | ||
# Make up Reaction Master mix (see table below). | # Make up Reaction Master mix (see table below). | ||
'''Table 1. Reaction Mix for full 96 well plate''' | |||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
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!5.25 ul | !5.25 ul | ||
!554.4 ul | !554.4 ul | ||
# Reaction Mix (5.25 ul) added to each well (see Table 2.) | |||
# Diluted gDNA samples each added to a single well (see Table 2.) | |||
'''Table 2. for full 96 well plate''' | |||
{| class="wikitable" | |||
|- | |||
! Component | |||
! 1X volume | |||
! 96X Volume (+ 10% excess) | |||
|- | |||
|Reaction Master Mix | |||
|5.25 ul | |||
|554.4 ul | |||
|- | |||
|gDNA samples | |||
|4.75 ul | |||
|501.6 ul | |||
|- | |||
! | |||
!10 ul | |||
!1056 ul | |||