TaqMan SNP Assay: Difference between revisions
No edit summary |
No edit summary |
||
| Line 15: | Line 15: | ||
== Protocol == | == Protocol == | ||
# Dilute gDNA samples to 1-5 ng/ul. | # Dilute gDNA samples to 1-5 ng/ul. | ||
# Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice. | # Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice. | ||
# Make up Reaction Master mix (see table below). | # Make up Reaction Master mix (see table below). | ||
{| class="wikitable" | |||
|- | |||
! Component | |||
! 1X volume | |||
! 96X Volume (+ 10% excess) | |||
|- | |||
|TaqMan® Genotyping Master Mix (2X) | |||
|TaqMan® Genotyping Assay (40X) | |||
|- | |||
|5.00 ul | |||
|0.25 ul | |||
|- | |||
|528.0 ul | |||
|26.4 ul | |||