Surveyor Assay for Genomic DNA Mutations: Difference between revisions
wrote initial protocol |
m changed volume of polymerase |
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* Using Platinum Taq High Fidelity amplify region of interest (design primers, should be <1000bp): | * Using Platinum Taq High Fidelity amplify region of interest (design primers, should be <1000bp): | ||
* Per reaction: | * Per reaction: | ||
** | ** 21.5 uL Platinum Taq High Fidelity Master Mix | ||
** 2.5 uL of Primers from a 2 uM stock solution | ** 2.5 uL of Primers from a 2 uM stock solution | ||
** 1 uL of the Extracted DNA from the previous step. | ** 1 uL of the Extracted DNA from the previous step. | ||