H&E Staining of Mouse Tissue: Difference between revisions
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# Place slide on edge and bake for 15 min at 60 degree C to melt wax | # Place slide on edge and bake for 15 min at 60 degree C to melt wax | ||
# Deparaffinize and rehydrate sections | # Deparaffinize and rehydrate sections | ||
## Immerse for 5 min with agitation in xylene | ## Immerse for 5 min with agitation in xylene/Citrisolv | ||
## Immerse for 5 min with agitation in 100% ethanol | ## Immerse for 5 min with agitation in 100% ethanol | ||
## Immerse for 5 min with agitation in 70% ethanol | ## Immerse for 5 min with agitation in 70% ethanol | ||
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#Place slides in 95% Ethanol two times for 30 seconds each. | #Place slides in 95% Ethanol two times for 30 seconds each. | ||
#Transfer slides into 100% Ethanol for 30 seconds, repeat with fresh 100% Ethanol. | #Transfer slides into 100% Ethanol for 30 seconds, repeat with fresh 100% Ethanol. | ||
#Wash twice with fresh xylene for 30 seconds. | #Wash twice with fresh xylene/Citrisolv for 30 seconds. | ||
#Let slide dry. | #Let slide dry. | ||
#Place 1-2 drops of mounting medium on slide and place coverslip over | #Place 1-2 drops of mounting medium on slide and place coverslip over | ||