H&E Staining of Mouse Tissue: Difference between revisions
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##depending on the intensity desired, repeat this step if necessary | ##depending on the intensity desired, repeat this step if necessary | ||
#Dip slide in 1% eosin Y fo 10-30 sec with agitation | #Dip slide in 1% eosin Y fo 10-30 sec with agitation | ||
##For adipose tissue, 20 seconds allows for proper staining. | |||
*Dehydration | *Dehydration | ||
#Place slides in 95% Ethanol two times for 30 seconds each. | #Place slides in 95% Ethanol two times for 30 seconds each. | ||
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#Place 1-2 drops of mounting medium on slide and place coverslip over | #Place 1-2 drops of mounting medium on slide and place coverslip over | ||
#Seal coverslip with sealant(nailpolish) | #Seal coverslip with sealant(nailpolish) | ||
==Notes== | ==Notes== | ||