Perfusion of Mouse: Difference between revisions
Created page with "==Materials== *0.9 % Saline solution *10% formalin solution *Avertin *Isofluorane *30 ½ gauge needle *1 mL syringe *Peristaltic pump *Collecting dish *Surgical stage/platform..." |
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**Do NOT use a solution that is yellow or contains a precipitate. | **Do NOT use a solution that is yellow or contains a precipitate. | ||
**Avertin is lipid soluble and may require a larger dose. | **Avertin is lipid soluble and may require a larger dose. | ||
==Perfusion Pump Set Up== | |||
*Place 30 ½ gauge on end of tubing | |||
*Insert one tube into 9% saline solution, and one tube into 10% formalin solution | |||
''Image 1: Set up of the two tube system from 0.9% saline solution and 10% formalin solution.'' | |||
[[File:perfusionbottle.png]] | |||
*Turn pump on to a flow rate of 0.72 ml/min | |||
*Have stopcock turned to allow for flow of saline | |||
[[File:perfusionstopcock.png]] | |||
*Fill line with 0.9% saline until you have a steady stream of 0.9% saline from the end of the needle. | |||
*Turn off peristaltic pump and turn stopcock to allow for formalin solution to flow | |||
*Turn on peristaltic pump and pump 10% formalin solution until flow fluid is before the stopcock | |||
**Flow has not entered the main line | |||
*Turn off peristaltic pump and turn stopcock to allow for saline to flow | |||
*Turn on pump and pump saline through the main line at stated flow rate | |||
''Image 3: Overview of the entire perfusion setup (excluding the procedural platform in the collecting dish)'' | |||