H&E Staining of Mouse Tissue: Difference between revisions
Created page with "==Materials== *xylene *100% Ethanol *70% Ethanol *H2O *Mayer's Hematoxylin *1% Eosin Y (in water) *95% Ethanol ==Protocol== *Removing Wax and Rehydrating # Place slide on ed..." |
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##depending on the intensity desired, repeat this step if necessary | ##depending on the intensity desired, repeat this step if necessary | ||
#Dip slide in 1% eosin Y fo 10-30 sec with agitation | #Dip slide in 1% eosin Y fo 10-30 sec with agitation | ||
*Dehydration | |||
#Place slides in 95% Ethanol two times for 30 seconds each. | |||
#Transfer slides into 100% Ethanol for 30 seconds, repeat with fresh 100% Ethanol. | |||
#Wash twice with fresh xylene for 30 seconds. | #Wash twice with fresh xylene for 30 seconds. | ||
#Let slide dry. | #Let slide dry. | ||