Primer Design for qPCR: Difference between revisions
added direction to primer name |
updated specifying to start with the ncbi gene page |
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==Making Your Own Primers From Entrez== | ==Making Your Own Primers From Entrez== | ||
# Find your RNA sequence on entrez by | # Find your RNA sequence on entrez by starting with the gene at http://www.ncbi.nlm.nih.gov/gene (make sure its the correct species). Then pick the mRNA you want to probe, based on the isoform structure on that page. Click on that nucleotide | ||
# Under analyse this sequence click '''Pick Primers''' | # Under analyse this sequence click '''Pick Primers''' | ||
# Under PCR Product Size pick 70-150 as the range | # Under PCR Product Size pick 70-150 as the range | ||