Triglyceride Assay from Cells and Tissues: Difference between revisions
Updated protocol for tissue culture |
Undo revision 853 by Davebridges (talk) |
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==Protocol== | ==Protocol== | ||
#Weigh out 30mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube. Add one stainless steel ball bearing. | #Weigh out 30mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube. Add one stainless steel ball bearing. | ||
#Add | #Add 500ul Homogenization Buffer | ||
#Homogenize | #Homogenize with Qiagen Tissue Lyser for 3 minutes @ 25Hz for Liver/WAT or for 5 minutes @ 30Hz for muscle | ||
#Add | #Add 12.5ul KOH | ||
#Mix by inverting | #Mix by inverting | ||
#Add 800ul '''Chloroform/Methanol Mixture''' | #Add 800ul '''Chloroform/Methanol Mixture''' | ||