French Press: Difference between revisions

imported protocol
 
Jpecherer (talk | contribs)
m Changes to protocol based on the non-functionality of the sample loading aparatus. More specific information on loading the cylinder into the French Press added.
 
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==Prepare the Cell (Not Auto-Fill)==
==Prepare the Cell (Not Auto-Fill)==
#Invert cell on black stand without base piece.  Remove piston to fill line
#Pour in sample, replace base and place back on the press.  Ensure valves are tightened
#Pour in sample, replace base and place back on the press.  Ensure valves are tightened


==Loading Sample==
==Loading Sample==
#With the back valve open and the bottom valve closed insert tubing into sample
#Set French Press to Down and start. Pour sample into cylinder followed by the piston.
#Set French Press to Down and start. Suction caused by the piston moving out of the cell will bring sample into the cell
#Ensure that the piston is lubricated with glycerol.
#When the base is at the bottom close the intake valve and stop the Press
#Place cylinder with piston into the French Press instrument from the back, making sure that the piston's lever is orthogonal to the brace screws.
#Position the valves such that they are accessible to your hands.


==Lysing Sample==
==Lysing Sample==