Splitting Cells: Difference between revisions
Created page with '==Materials== *FBS (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM *PBS -/- *0.05% Trypsin ==Protocol== #W...' |
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==Materials== | ==Materials== | ||
* | *Media (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM | ||
*PBS -/- | *PBS -/- | ||
*0.05% Trypsin | *0.05% Trypsin | ||
==Protocol== | ==Protocol== | ||
#Warm PBS and Media | #Warm PBS and Media in water bath | ||
#Wash cells twice with 10 mL (per 10 cm dish) PBS -/- | #Wash cells twice with 10 mL (per 10 cm dish) PBS -/- | ||
#Add 1 mL trypsin and sit in the hood | #Add 1 mL trypsin and sit in the hood | ||