Transformation of Bacteria: Difference between revisions
removed confusing buffer addition |
updated transformation protocol |
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==Materials== | ==Materials== | ||
*Competent Cells | *Competent Cells | ||
*SOC Buffer or LB Media | |||
*SOC Buffer | |||
*DNA of interest (1 uL for plasmid, 5 uL for cloning/mutagenesis) | *DNA of interest (1 uL for plasmid, 5 uL for cloning/mutagenesis) | ||
*Plates (Amp or Kan; in cold room) | *Plates (Amp or Kan; in cold room, see [[ Making LB Agar Plates ]]) | ||
==Protocol== | ==Protocol== | ||