Differentiation of MEFs: Difference between revisions

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~~'''~~==Protocol==~~'''~~Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)

==Protocol==

~~'''~~==Strains==~~'''~~ Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs

Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)

~~'''~~==Source==~~'''~~ DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)

==Strains==

~~'''~~==Papers==~~'''~~

Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs

==Source==

DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)

==Papers==

• Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.

• Zhang H, Cicchetti G, Onda H, Koon HB, Asrican K, et al. (2003) Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112: 1223–1233.

@@ Line 1: / Line 1: @@
-'''==Protocol=='''Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
+==Protocol==
-'''==Strains==''' Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs
+Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
-'''==Source==''' DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
+==Strains==
-'''==Papers=='''
+Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs
+==Source==
+DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
+==Papers==
 •	Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.
 •	Zhang H, Cicchetti G, Onda H, Koon HB, Asrican K, et al. (2003) Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112: 1223–1233.