Differentiation of MEFs: Difference between revisions

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Protocol: Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)

==Protocol==

Strains: Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs

Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)

Source: DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)

==Strains==

Papers:

Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs

==Source==

DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)

==Papers==

• Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.

• Zhang H, Cicchetti G, Onda H, Koon HB, Asrican K, et al. (2003) Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112: 1223–1233.

Materials

==Materials==

• DMEM (4.5 g/L glucose + L-glutamine – sodium pyruvate; Invitrogen catalog # 11965-092)

• FBS (we test several batches from several sources for differentiation and insulin stimulated glucose uptake then order large quantities of that particular lot. Currently we are using Sigma cat# F2442, lot# 072K8403. Aliquot and store in 50 mL tubes at -20)

@@ Line 1: / Line 1: @@
-Protocol: Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
+==Protocol==
-Strains: Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs
+Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
-Source: DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
+==Strains==
-Papers:
+Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs
+==Source==
+DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
+==Papers==
 •	Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.
 •	Zhang H, Cicchetti G, Onda H, Koon HB, Asrican K, et al. (2003) Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112: 1223–1233.
-Materials
+==Materials==
 •	DMEM (4.5 g/L glucose + L-glutamine – sodium pyruvate; Invitrogen catalog # 11965-092)
 •	FBS (we test several batches from several sources for differentiation and insulin stimulated glucose uptake then order large quantities of that particular lot. Currently we are using Sigma cat# F2442, lot# 072K8403. Aliquot and store in 50 mL tubes at -20)