Differentiation of MEFs: Difference between revisions

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'''==Protocol=='''Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
==Protocol==Adipogenic Differentiation of Mouse Embryonic Fibroblasts (MEFs)
'''==Strains==''' Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs  
==Strains==Wild type (Tsc2 +/+) and Tsc2 -/- (p53−/−) MEFs  
'''==Source==''' DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
==Source==DJ Kwiatkowski (Brigham and Women's Hospital, Boston, MA)
==Papers==  
==Papers==  
• Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.  
• Kwiatkowski DJ, Zhang H, Bandura JL, Heiberger KM, Glogauer M, et al. (2002) A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in TSC1 null cells. Hum Mol Genet 11: 525–534.