Difference between revisions of "Transfer Buffer"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (copied recipe from saltiel lab buffers) |
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Revision as of 14:57, 12 April 2013
10X Transfer Buffer
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
- 100 mL 10X Buffer
- 200 mL Methanol
- 700 mL Water
| Component | Weight per 10 L | Catalog Number |
|---|---|---|
| Tris Base | 303 g | Sigma T6066-5KG |
| Glycine | 1441 g | Sigma G7126-5KG |
