Lipofectamine Transfection of C2C12 Cells: Difference between revisions
Wrote protocol from ethan's trials |
m changed confluence note |
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==Materials== | ==Materials== | ||
*Cells in a 6 well dish, plated and at | *Cells in a 6 well dish, plated and at ~70% confluence. Typically transfect as myocytes. | ||
*Lipofectamine 2000 (Invitrogen cat# 11668-019 for 1.5mL) | *Lipofectamine 2000 (Invitrogen cat# 11668-019 for 1.5mL) | ||
*OptiMEM | *OptiMEM | ||