Transformation of Bacteria: Difference between revisions
m Update |
removed confusing buffer addition |
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| Line 14: | Line 14: | ||
#Heat shock at 42C for 45s | #Heat shock at 42C for 45s | ||
#Place back on ice | #Place back on ice | ||
#Add 450 uL of SOC Buffer or LB | #Add 450 uL of SOC Buffer or LB. | ||
#Incubate at 37C for 1h with occasional mixing | #Incubate at 37C for 1h with occasional mixing | ||
#Plate 30-50 uL for amplification, or all for cloning/mutagenesis | #Plate 30-50 uL for amplification, or all for cloning/mutagenesis | ||
*When plating be sure to label plates and use extreme caution when sterilizing the spreader with EtOH and fire. | *When plating be sure to label plates and use extreme caution when sterilizing the spreader with EtOH and fire. | ||