Immunofluoresence: Difference between revisions
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==Reagents== | ==Reagents== | ||
*Fixative: Neutral buffered formalin or 4% Paraformaldehyde (for | *Fixative: Neutral buffered formalin or 4% Paraformaldehyde (for lipid/membrane bound proteins) in PBS or ice cold 10% methanol (for cytoskeleton bound proteins) | ||
*Cold PBS | *Cold PBS | ||
*100 mM Glycine in PBS | *100 mM Glycine in PBS | ||