Triglyceride Assay from Cells and Tissues: Difference between revisions
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==Materials== | ==Materials== | ||
* '''Homogenization Buffer''' (50 mM Tris, 5 mM EDTA, 30 mM Mannitol, PI inhibitor, can be made in bulk without the PI, PI added fresh) | * '''Homogenization Buffer''' (50 mM Tris pH 8, 5 mM EDTA, 30 mM Mannitol, PI inhibitor, can be made in bulk without the PI, PI added fresh) | ||
* 10M KOH | * 10M KOH | ||
* '''Chloroform/Methanol Mixture''' (2:1) | * '''Chloroform/Methanol Mixture''' (2:1) | ||