Lipofectamine Plasmid Transfection: Difference between revisions
No edit summary |
No edit summary |
||
| Line 21: | Line 21: | ||
#Add DNA/Lipofectamine complexes (~500 uL) to the 2 mL of media on the cells | #Add DNA/Lipofectamine complexes (~500 uL) to the 2 mL of media on the cells | ||
#Gently rock plate to mix | #Gently rock plate to mix | ||
#After ~4h re-feed cells with normal media. | #After ~4h aspirate media and re-feed cells with normal media. (It is usually possible to leave overnight). | ||
Protocol adapted from [http://tools.invitrogen.com/content/sfs/manuals/lipofectamine2000_man.pdf Product Manual] | Protocol adapted from [http://tools.invitrogen.com/content/sfs/manuals/lipofectamine2000_man.pdf Product Manual] | ||